Regulatory

Part:BBa_K1163400

Designed by: Emiel van der Kouwe   Group: iGEM13_Evry   (2013-09-22)


AceB promoter, iron sensitive, down-regulated by dimerized FUR protein

Promoter from E. coli that controls the AceB gene, involved in iron uptake.

This promoter sequence contains a RBS and a FUR binding site, although it is not clearly possible to identify them precisely. It has been shown to downregulate the expression of sfGFP or any gene down-stream in the presence or iron in the range 10^-7, 10^-5 mol.L-1

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 277
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None